Review



visium spatial gene expression 30 library construction v1 kit  (10X Genomics)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    10X Genomics visium spatial gene expression 30 library construction v1 kit
    Visium Spatial Gene Expression 30 Library Construction V1 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/expression+gene+slides+spatial+visium/10__1016_slash_j__celrep__2024__115123-241-27-36
    Average 86 stars, based on 1 article reviews
    visium spatial gene expression 30 library construction v1 kit - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Gene Expression:

    Article Title: Single-cell and spatial transcriptomics identify a macrophage population associated with skeletal muscle fibrosis.
    Article Snippet: Following imaging, tissues were permeabilized for 12 min, which was predetermined as the optimal time for 10-μm mouse muscle sections using the 10x Genomics Visium Tissue Optimization Kit. .. Spatially tagged cDNA libraries were built using the 10x Genomics Visium Spatial Gene Expression Library Construction Kit. .. Sequencing was performed on an Illumina NextSeq 500/550 using 150-cycle high output kits (read 1 = 28, read 2 = 120, index 1 = 10, and index 2 = 10).

    Article Title: Single-cell and spatial transcriptomics identify a macrophage population associated with skeletal muscle fibrosis
    Article Snippet: Following imaging, tissues were permeabilized for 12 minutes, which was predetermined as the optimal time for 10 μm mouse muscle sections using the 10x Genomics Visium Tissue Optimization Kit. .. Spatially-tagged cDNA libraries were built using the 10x Genomics Visium Spatial Gene Expression Library Construction Kit. .. Sequencing was performed on an Illumina NextSeq 500/550 using 150-cycle High Output kits (Read 1 = 28, Read 2 = 120, Index 1 = 10, and Index 2 = 10).

    Article Title: Spatial programming of fibroblasts promotes resolution of tissue inflammation through immune cell exclusion
    Article Snippet: Each capture area was imaged using Axio Z1 Slide Scanner (Zeiss). .. Permeabilisation of the synovial tissue samples was performed for a total of 12 minutes at 37 °C, and libraries were constructed using the Visium Spatial Gene Expression Library Construction Kit (PN-1000186 and PN-1000190, 10x Genomics), according to manufacturers’ instructions. .. Library quantification was carried out using KAPA SYBR FAST kit (KK4600, Sigma-Aldrich) and sequenced by Genomics Birmingham (Birmingham, UK) using a high output reagent Dual Index Kit TT Set A (10x Genomics) and the NextSeq500 machine (Illumina).

    Construct:

    Article Title: Spatial programming of fibroblasts promotes resolution of tissue inflammation through immune cell exclusion
    Article Snippet: Each capture area was imaged using Axio Z1 Slide Scanner (Zeiss). .. Permeabilisation of the synovial tissue samples was performed for a total of 12 minutes at 37 °C, and libraries were constructed using the Visium Spatial Gene Expression Library Construction Kit (PN-1000186 and PN-1000190, 10x Genomics), according to manufacturers’ instructions. .. Library quantification was carried out using KAPA SYBR FAST kit (KK4600, Sigma-Aldrich) and sequenced by Genomics Birmingham (Birmingham, UK) using a high output reagent Dual Index Kit TT Set A (10x Genomics) and the NextSeq500 machine (Illumina).



    Similar Products

    visium spatial gene expression 30 library construction v1 kit  (10X Genomics)
    86
    10X Genomics visium spatial gene expression 30 library construction v1 kit
    Visium Spatial Gene Expression 30 Library Construction V1 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/expression+gene+slides+spatial+visium/10__1016_slash_j__celrep__2024__115123-241-27-36
    Average 86 stars, based on 1 article reviews
    visium spatial gene expression 30 library construction v1 kit - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    visium spatial gene expression library construction kit  (10X Genomics)
    90
    10X Genomics visium spatial gene expression library construction kit
    a) Schematic overview of multi-modal study design, including patient cohort, sample collection and processing for spatial transcriptomics <t>(ST;</t> <t>10x</t> Genomics <t>Visium)</t> and multiplex immunofluorescence (IF; Leica Cell DIVE). b) Representative spatial plot showing a section of annotated spots from ST of synovial tissue with matched H&E stain. c) UMAP representation of ST spots, annotated based on gene expression. d) Dotplot of key marker genes and GO terms that define spatial tissue niches. e) Spatial plot of deconvoluted spots in synovial tissue following BayesSpace analysis. f) Heatmap showing presence of cell types in tisue niches following BayesSpace analysis based on scRNA-seq reference datasets (Zhang et al., 2019; Wei et al., 2020). g) Representative spatial plot (I) and matched H&E-stained synovial tissue (II) with spatial plots showing expression of gene modules that define tissue niches (III). h) Representative multiplex IF image of synovial tissue showing markers that define tissue architecture. Scale bar: 100 µm. i) Matched representative tissue image following computational cell segmentation showing cell centroids and annotation of ST-defined tissue niches. j) Comparison of marker gene expression between niches in IF and ST data. k) Representative multiplex IF images showing key markers of each niche as well as PDPN, CD68 and CD31 to mark major tissue landmarks. Plasma cell niche imaged using PhenoImager™ HT (Akoya Biosciences); all other niches imaged using Cell DIVE (Leica). Scale bars: 100 µm.
    Visium Spatial Gene Expression Library Construction Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/visium+spatial+gene+expression/bio_rxiv__2024__09__20__614064-183-23-33
    Average 90 stars, based on 1 article reviews
    visium spatial gene expression library construction kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    visium spatial gene expression 3 library construction v1 kit  (10X Genomics)
    86
    10X Genomics visium spatial gene expression 3 library construction v1 kit
    a) Schematic overview of multi-modal study design, including patient cohort, sample collection and processing for spatial transcriptomics <t>(ST;</t> <t>10x</t> Genomics <t>Visium)</t> and multiplex immunofluorescence (IF; Leica Cell DIVE). b) Representative spatial plot showing a section of annotated spots from ST of synovial tissue with matched H&E stain. c) UMAP representation of ST spots, annotated based on gene expression. d) Dotplot of key marker genes and GO terms that define spatial tissue niches. e) Spatial plot of deconvoluted spots in synovial tissue following BayesSpace analysis. f) Heatmap showing presence of cell types in tisue niches following BayesSpace analysis based on scRNA-seq reference datasets (Zhang et al., 2019; Wei et al., 2020). g) Representative spatial plot (I) and matched H&E-stained synovial tissue (II) with spatial plots showing expression of gene modules that define tissue niches (III). h) Representative multiplex IF image of synovial tissue showing markers that define tissue architecture. Scale bar: 100 µm. i) Matched representative tissue image following computational cell segmentation showing cell centroids and annotation of ST-defined tissue niches. j) Comparison of marker gene expression between niches in IF and ST data. k) Representative multiplex IF images showing key markers of each niche as well as PDPN, CD68 and CD31 to mark major tissue landmarks. Plasma cell niche imaged using PhenoImager™ HT (Akoya Biosciences); all other niches imaged using Cell DIVE (Leica). Scale bars: 100 µm.
    Visium Spatial Gene Expression 3 Library Construction V1 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/expression+gene+slides+spatial+visium/pmc11342764-81-12-10
    Average 86 stars, based on 1 article reviews
    visium spatial gene expression 3 library construction v1 kit - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    10x genomics visium spatial gene expression 30 library construction v1 kit  (10X Genomics)
    90
    10X Genomics 10x genomics visium spatial gene expression 30 library construction v1 kit
    a) Schematic overview of multi-modal study design, including patient cohort, sample collection and processing for spatial transcriptomics <t>(ST;</t> <t>10x</t> Genomics <t>Visium)</t> and multiplex immunofluorescence (IF; Leica Cell DIVE). b) Representative spatial plot showing a section of annotated spots from ST of synovial tissue with matched H&E stain. c) UMAP representation of ST spots, annotated based on gene expression. d) Dotplot of key marker genes and GO terms that define spatial tissue niches. e) Spatial plot of deconvoluted spots in synovial tissue following BayesSpace analysis. f) Heatmap showing presence of cell types in tisue niches following BayesSpace analysis based on scRNA-seq reference datasets (Zhang et al., 2019; Wei et al., 2020). g) Representative spatial plot (I) and matched H&E-stained synovial tissue (II) with spatial plots showing expression of gene modules that define tissue niches (III). h) Representative multiplex IF image of synovial tissue showing markers that define tissue architecture. Scale bar: 100 µm. i) Matched representative tissue image following computational cell segmentation showing cell centroids and annotation of ST-defined tissue niches. j) Comparison of marker gene expression between niches in IF and ST data. k) Representative multiplex IF images showing key markers of each niche as well as PDPN, CD68 and CD31 to mark major tissue landmarks. Plasma cell niche imaged using PhenoImager™ HT (Akoya Biosciences); all other niches imaged using Cell DIVE (Leica). Scale bars: 100 µm.
    10x Genomics Visium Spatial Gene Expression 30 Library Construction V1 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/10x+genomics+visium+spatial+gene+expression+3%E2%80%99+library+construction+v1+kit/pm39096826-67-12-10
    Average 90 stars, based on 1 article reviews
    10x genomics visium spatial gene expression 30 library construction v1 kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    visium spatial gene expression library construction v1 kit  (10X Genomics)
    90
    10X Genomics visium spatial gene expression library construction v1 kit

    Visium Spatial Gene Expression Library Construction V1 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/visium+spatial+gene+expression+3%E2%80%99+library+construction+v1+kit/pmc11298649-44-0-9
    Average 90 stars, based on 1 article reviews
    visium spatial gene expression library construction v1 kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    visium spatial gene expression 3' library construction v1 kit  (10X Genomics)
    90
    10X Genomics visium spatial gene expression 3' library construction v1 kit

    Visium Spatial Gene Expression 3' Library Construction V1 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/visium+spatial+gene+expression+3%E2%80%99+library+construction+v1+kit/pmc10468137-277-10-8
    Average 90 stars, based on 1 article reviews
    visium spatial gene expression 3' library construction v1 kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    visium spatial gene expression 3 library construction kit  (10X Genomics)
    86
    10X Genomics visium spatial gene expression 3 library construction kit

    Visium Spatial Gene Expression 3 Library Construction Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+library+construction+kit/expression+gene+slides+spatial+visium/pm37202489-468-38-49
    Average 86 stars, based on 1 article reviews
    visium spatial gene expression 3 library construction kit - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    a) Schematic overview of multi-modal study design, including patient cohort, sample collection and processing for spatial transcriptomics (ST; 10x Genomics Visium) and multiplex immunofluorescence (IF; Leica Cell DIVE). b) Representative spatial plot showing a section of annotated spots from ST of synovial tissue with matched H&E stain. c) UMAP representation of ST spots, annotated based on gene expression. d) Dotplot of key marker genes and GO terms that define spatial tissue niches. e) Spatial plot of deconvoluted spots in synovial tissue following BayesSpace analysis. f) Heatmap showing presence of cell types in tisue niches following BayesSpace analysis based on scRNA-seq reference datasets (Zhang et al., 2019; Wei et al., 2020). g) Representative spatial plot (I) and matched H&E-stained synovial tissue (II) with spatial plots showing expression of gene modules that define tissue niches (III). h) Representative multiplex IF image of synovial tissue showing markers that define tissue architecture. Scale bar: 100 µm. i) Matched representative tissue image following computational cell segmentation showing cell centroids and annotation of ST-defined tissue niches. j) Comparison of marker gene expression between niches in IF and ST data. k) Representative multiplex IF images showing key markers of each niche as well as PDPN, CD68 and CD31 to mark major tissue landmarks. Plasma cell niche imaged using PhenoImager™ HT (Akoya Biosciences); all other niches imaged using Cell DIVE (Leica). Scale bars: 100 µm.

    Journal: bioRxiv

    Article Title: Spatial programming of fibroblasts promotes resolution of tissue inflammation through immune cell exclusion

    doi: 10.1101/2024.09.20.614064

    Figure Lengend Snippet: a) Schematic overview of multi-modal study design, including patient cohort, sample collection and processing for spatial transcriptomics (ST; 10x Genomics Visium) and multiplex immunofluorescence (IF; Leica Cell DIVE). b) Representative spatial plot showing a section of annotated spots from ST of synovial tissue with matched H&E stain. c) UMAP representation of ST spots, annotated based on gene expression. d) Dotplot of key marker genes and GO terms that define spatial tissue niches. e) Spatial plot of deconvoluted spots in synovial tissue following BayesSpace analysis. f) Heatmap showing presence of cell types in tisue niches following BayesSpace analysis based on scRNA-seq reference datasets (Zhang et al., 2019; Wei et al., 2020). g) Representative spatial plot (I) and matched H&E-stained synovial tissue (II) with spatial plots showing expression of gene modules that define tissue niches (III). h) Representative multiplex IF image of synovial tissue showing markers that define tissue architecture. Scale bar: 100 µm. i) Matched representative tissue image following computational cell segmentation showing cell centroids and annotation of ST-defined tissue niches. j) Comparison of marker gene expression between niches in IF and ST data. k) Representative multiplex IF images showing key markers of each niche as well as PDPN, CD68 and CD31 to mark major tissue landmarks. Plasma cell niche imaged using PhenoImager™ HT (Akoya Biosciences); all other niches imaged using Cell DIVE (Leica). Scale bars: 100 µm.

    Article Snippet: Permeabilisation of the synovial tissue samples was performed for a total of 12 minutes at 37 °C, and libraries were constructed using the Visium Spatial Gene Expression Library Construction Kit (PN-1000186 and PN-1000190, 10x Genomics), according to manufacturers’ instructions.

    Techniques: Multiplex Assay, Immunofluorescence, Staining, Expressing, Marker, Comparison

    Journal: iScience

    Article Title: Respiratory infection with influenza A virus delays remyelination and alters oligodendrocyte metabolism

    doi: 10.1016/j.isci.2024.110464

    Figure Lengend Snippet:

    Article Snippet: Visium Spatial Gene Expression Library Construction v1 Kit , 10x Genomics , Cat# PN-1000187.

    Techniques: Virus, Recombinant, Gene Expression, Software